False-negative Dengue Cases
نویسندگان
چکیده
Dear Editor, we would like to discuss on the recent publication on false-negative dengue cases. ACOSTA et al. reported that “Health care providers should therefore be aware of samples tested negative by NS1 antigen assays, especially when clinical symptoms and other laboratory data results show evidence of dengue infection”. In fact, the problem of false-negative in dengue diagnosis is an important issue for discussion. First, the problem of the NS1 assay in dengue diagnosis is noted worldwide. Combining with other immunological test is suggested for improvement of diagnostic accuracy. Also, it should be noted that the diagnosis of dengue in many tropical countries is usually a presumptive diagnosis without the laboratory confirmation. This implies that there can be both false-positive and false-negative of dengue diagnosis. Sometimes, other arboviral infection such as Chikungunya virus infection can have the similar clinical feature to dengue and mis-diagnosed as dengue. In addition, there can be more than one arboviral infection in a case (such as co-infection between dengue and Mayaro virus infection). Nevertheless, the definitive diagnosis is more useful for epidemiological purpose than therapeutic purpose. The symptomatic and supportive treatment is usually the basic effective management for the patient with dengue and other similar arboviral infections.
منابع مشابه
False-negative Dengue Cases in Roraima, Brazil: an Approach regarding the High Number of Negative Results by Ns1 Ag Kits
Serum samples from 150 NS1-negative (Platelia ELISA) patients presumptively diagnosed with dengue were analyzed by the TaqMan probed real-time reverse transcription PCR (TaqMan qRT-PCR) method. The qRT-PCR positive samples were tested for serotype by semi-nested RT-PCR and a qualitative immunochromatographic assay for IgG and IgM. Molecular detection methods showed 33 (22%) positive samples out...
متن کاملSerological Diagnostic Assays for Detection of Ns1 Antigen, IGM and IGG Antbodies to Dengue Virus
Complications in managing the dengue virus infections include the lack of rapid diagnostic procedures and at the same time the symptoms of dengue infection are often confused with those of other diseases. Two commercial rapid serological diagnostic kit methods (Dengue Day 1 test, J Mitra and Co. Pvt. Ltd., New Delhi, ImmunoComb II Dengue IgM/IgGBispot kit (Orgenics Pvt. Ltd., Israel)were evalua...
متن کاملLow sensitivity of NS1 protein tests evidenced during a dengue type 2 virus outbreak in Santos, Brazil, in 2010.
In 2010, a large outbreak of dengue occurred in Santos, Brazil. The detection of the NS1 antigen was used for diagnosis in addition to the detection of IgG, IgM, and RNA. A large number of NS1 false-negative results were obtained. A total of 379 RNA-positive samples were selected for thorough evaluation. NS1 was reactive in 37.7% of cases. Most of the cases were characterized as a secondary inf...
متن کاملRepeated tourniquet testing as a diagnostic tool in dengue infection.
Prospective evaluation of repeated standard tourniquet testing as a diagnostic indicator of dengue infection was done. Included were patients admitted to a children's hospital in Kuala Lumpur on a clinical suspicion of dengue infection based on the World Health Organization (WHO) criteria. A standard method of tourniquet was performed on 79 patients on a daily basis following admission. subject...
متن کامل